Hotspot

rpl20-rps12

LSC · 65670-66481 · 812 bp

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Pi 0.0484

MarkerSeek score 85.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 812
variable_sites: 111
pi: 0.0484
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	ATCCGCGTTTAATCCTGGTC	CGAGGAACATGTACTAGGGTG	50.0	52.4	58.1	57.6	850-860 bp	1.0	80.0
rpl20-rps12_p2	CCGCGTTTAATCCTGGTCAT	CGAGGAACATGTACTAGGGTG	50.0	52.4	58.3	57.6	848-858 bp	1.0	77.7
rpl20-rps12_p3	ATCCGCGTTTAATCCTGGTC	CGAGGAACATGTACTAGGGT	50.0	50.0	58.1	56.1	850-860 bp	1.0	76.1
rpl20-rps12_p4	CCGCGTTTAATCCTGGTCAT	CGAGGAACATGTACTAGGGT	50.0	50.0	58.3	56.1	848-858 bp	1.0	73.8
rpl20-rps12_p5	TATCCGCGTTTAATCCTGGTC	CGAGGAACATGTACTAGGGTG	47.6	52.4	57.9	57.6	851-861 bp	1.0	71.6