Hotspot

petA-psbJ

LSC · 60039-61010 · 972 bp

Back to Genlisea

Pi 0.0721

MarkerSeek score 83.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 972
variable_sites: 185
pi: 0.0721
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CAATTGGCCGAAATGAATTTCT	GGATTAGGTTCATCCCTGTAG	36.4	47.6	56.2	54.9	930-1057 bp	1.0	52.0
petA-psbJ_p2	CAATTGGCCGAAATGAATTTCT	GGATTAGGTTCATCCCTGTA	36.4	45.0	56.2	53.5	930-1057 bp	1.0	52.0
petA-psbJ_p3	ATTGGCCGAAATGAATTTCT	GGATTAGGTTCATCCCTGTAG	35.0	47.6	53.8	54.9	928-1055 bp	1.0	52.0
petA-psbJ_p4	CAATTGGCCGAAATGAATTTC	GGATTAGGTTCATCCCTGTAG	38.1	47.6	54.7	54.9	930-1057 bp	1.0	52.0
petA-psbJ_p5	AATTGGCCGAAATGAATTTCT	GGATTAGGTTCATCCCTGTAG	33.3	47.6	54.7	54.9	929-1056 bp	1.0	52.0