Hotspot

atpB-rbcL

LSC · 51567-52368 · 802 bp

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Pi 0.0594

MarkerSeek score 85.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 802
variable_sites: 138
pi: 0.0594
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	ATTAGGCATCTTTCCTGGCG	GTAAGTCCATCGGTCCACAC	50.0	55.0	58.0	58.0	1151-1173 bp	1.0	91.0
atpB-rbcL_p2	ATTAGGCATCTTTCCTGGCG	AGAAACGATCTCTCCAACGC	50.0	50.0	58.0	58.0	1582-1604 bp	1.0	90.2
atpB-rbcL_p3	ATTAGGCATCTTTCCTGGCG	GTACCCGCAGTAGCATTCAA	50.0	50.0	58.0	58.0	1664-1686 bp	1.0	89.9
atpB-rbcL_p4	GTCTAGTACCGGACCGATGA	GTAAGTCCATCGGTCCACAC	55.0	55.0	58.0	58.0	1122-1143 bp	0.857143	80.7
atpB-rbcL_p5	GTCTAGTACCGGACCGATGA	AGAAACGATCTCTCCAACGC	55.0	50.0	58.0	58.0	1553-1574 bp	0.857143	79.6