Hotspot

psaA-ycf3

LSC · 41150-42116 · 967 bp

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Pi 0.0942

MarkerSeek score 76.60

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 967
variable_sites: 226
pi: 0.0942
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGCGAGTTGGATCACTTAGC	CGCGGAAGTTTGGTTTGATC	50.0	50.0	58.0	58.0	1241-1405 bp	1.0	91.6
psaA-ycf3_p2	TGCGAGTTGGATCACTTAGC	ATCAAGCCGCTCAGTATTGG	50.0	50.0	58.0	58.0	1224-1388 bp	1.0	91.6
psaA-ycf3_p3	ACATGTACTTGATGACCCGC	CGCGGAAGTTTGGTTTGATC	50.0	50.0	58.0	58.0	1569-1732 bp	0.857143	85.8
psaA-ycf3_p4	ACATGTACTTGATGACCCGC	ATCAAGCCGCTCAGTATTGG	50.0	50.0	58.0	58.0	1552-1715 bp	0.857143	85.8
psaA-ycf3_p5	TGCGAGTTGGATCACTTAGC	CAATTTGCGAGGTTTGGGTC	50.0	50.0	58.0	57.9	1871-1953 bp	0.714286	74.4