Hotspot

trnT-GGU-psbD

LSC · 30999-32235 · 1237 bp

Back to Genlisea

Pi 0.068
MarkerSeek score 84.30
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1237
variable_sites
234
pi
0.068
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GCCCTTTTAACTCAGCGGTA AAAGGTTGTACCCGTGAACC 50.0 50.0 57.9 58.0 1475-1498 bp 0.857143 83.8
trnT-GGU-psbD_p2 GCCCTTTTAACTCAGCGGTA TGGACCAGAGAATGCGATTG 50.0 50.0 57.9 58.0 1760-1783 bp 0.857143 82.4
trnT-GGU-psbD_p3 GCCCTTTTAACTCAGCGGTA AAAAGGGTTCAGCGTCCAAT 50.0 45.0 57.9 58.0 1901-1924 bp 0.857143 82.0
trnT-GGU-psbD_p4 GCCCTTTTAACTCAGCGGTA AAAGGGTTCAGCGTCCAATT 50.0 45.0 57.9 58.0 1900-1923 bp 0.857143 82.0
trnT-GGU-psbD_p5 GCCCTTTTAACTCAGCGGTA GCGGTGACCATGGAATAAGT 50.0 50.0 57.9 58.0 2059-2082 bp 0.714286 70.1