Hotspot

trnS-GCU-trnG-UCC

LSC · 8112-8819 · 708 bp

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Pi 0.0896
MarkerSeek score 84.40
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
708
variable_sites
164
pi
0.0896
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TCCACTCAGCCATCTCTCC ACCACTAAACTATACCCGCT 57.9 45.0 58.1 55.6 614-773 bp 1.0 64.4
trnS-GCU-trnG-UCC_p2 GTCCACTCAGCCATCTCTCC ACCACTAAACTATACCCGCT 60.0 45.0 59.5 55.6 615-774 bp 1.0 60.9
trnS-GCU-trnG-UCC_p3 GTCCACTCAGCCATCTCTC ACCACTAAACTATACCCGCT 57.9 45.0 56.9 55.6 615-774 bp 1.0 56.7
trnS-GCU-trnG-UCC_p4 TCCACTCAGCCATCTCTCC TACCACTAAACTATACCCGCT 57.9 42.9 58.1 55.6 615-774 bp 1.0 56.1
trnS-GCU-trnG-UCC_p5 TCCACTCAGCCATCTCTCC ACGAATCACACTTTTACCACT 57.9 38.1 58.1 55.5 629-788 bp 1.0 55.0