Hotspot

trnK-UUU-rps16

LSC · 4206-5030 · 825 bp

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Pi 0.101

MarkerSeek score 83.80

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 825
variable_sites: 222
pi: 0.101
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	GTGTTGTTGAAATCGCTCGG	GCAATTGATGTTCGAGCTCG	50.0	50.0	58.0	57.9	1770-1795 bp	0.714286	74.8
trnK-UUU-rps16_p2	GTGTTGTTGAAATCGCTCGG	TTTTCTTGAGCCGTACGAGG	50.0	50.0	58.0	57.9	1866-1891 bp	0.714286	74.6
trnK-UUU-rps16_p3	TGAAATCGCTCGGGCTTTAA	TTTTCTTGAGCCGTACGAGG	45.0	50.0	57.9	57.9	1859-1879 bp	0.285714	50.6
trnK-UUU-rps16_p4	TTGAAATCGCTCGGGCTTTA	TTTTCTTGAGCCGTACGAGG	45.0	50.0	57.9	57.9	1860-1880 bp	0.285714	50.6
trnK-UUU-rps16_p5	TGAAATCGCTCGGGCTTTAA	GCAATTGATGTTCGAGCTCG	45.0	50.0	57.9	57.9	1763-1782 bp	0.285714	50.5