Hotspot

ycf1

SSC · 107959-113289 · 5331 bp

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Pi 0.0161

MarkerSeek score 48.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5331
variable_sites: 128
pi: 0.0161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCAATGAACGTCATGTGGCT	GGATTGGTATGATCCACGGG	45.0	55.0	57.8	57.8	474-501 bp	1.0	83.4
ycf1_p2	TCAATGAACGTCATGTGGCT	TTTGGCTTGGTTTCGAGTGT	45.0	45.0	57.8	58.2	620-647 bp	1.0	82.2
ycf1_p3	TGCGGAAAGGGCTGATAAAA	GGATTGGTATGATCCACGGG	45.0	55.0	57.8	57.8	875-902 bp	1.0	81.9
ycf1_p4	TCAATGAACGTCATGTGGCT	TGTCTTGTACGATTCTGCCG	45.0	50.0	57.8	58.0	719-737 bp	0.666667	67.2
ycf1_p5	TGCGGAAAGGGCTGATAAAA	TGTCTTGTACGATTCTGCCG	45.0	50.0	57.8	58.0	1120-1138 bp	0.666667	66.3