Hotspot

ndhI-ndhG

SSC · 118098-118458 · 361 bp

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Pi 0.0369

MarkerSeek score 66.30

Primer available yes

Label ndhI-ndhG

Feature Metadata

label_name: ndhI-ndhG
feature_type: igs
region: SSC
length_bp: 361
variable_sites: 20
pi: 0.0369
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhG_p1	AATCAAATTGCTTTGGGCCG	ATGTAGCGGTTCAGGCAAAT	45.0	45.0	57.9	57.9	460-484 bp	1.0	81.9
ndhI-ndhG_p2	TTTGGGCCGTTTACCAATGT	ATGTAGCGGTTCAGGCAAAT	45.0	45.0	58.3	57.9	449-473 bp	1.0	80.5
ndhI-ndhG_p3	AATCAAATTGCTTTGGGCCG	AATCATGTAGCGGTTCAGGC	45.0	50.0	57.9	58.3	464-488 bp	1.0	80.3
ndhI-ndhG_p4	CTTTGGGCCGTTTACCAATG	ATGTAGCGGTTCAGGCAAAT	50.0	45.0	57.6	57.9	450-474 bp	1.0	79.9
ndhI-ndhG_p5	CAAATTGCTTTGGGCCGTTT	ATGTAGCGGTTCAGGCAAAT	45.0	45.0	58.4	57.9	457-481 bp	1.0	79.5