Hotspot

trnH-rpl2

LSC · 82641-82755 · 115 bp

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Pi 0.0232

MarkerSeek score 66.20

Primer available yes

Label trnH-rpl2

Feature Metadata

label_name: trnH-rpl2
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 4
pi: 0.0232
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-rpl2_p1	CGGATGTAGCCAAGTGGATC	TGCGTCGCCGTAGTAAATAG	55.0	50.0	58.1	57.9	207-215 bp	1.0	80.0
trnH-rpl2_p2	TGGATCAAGGCAGTGGATTG	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.9	58.1	292-300 bp	1.0	79.9
trnH-rpl2_p3	CGGATGTAGCCAAGTGGATC	GGGCCCCAATTGGTAGAAAA	55.0	50.0	58.1	58.1	306-314 bp	1.0	79.8
trnH-rpl2_p4	GGATCAAGGCAGTGGATTGT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.9	58.1	291-299 bp	1.0	79.8
trnH-rpl2_p5	GTGGATCAAGGCAGTGGATT	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.9	58.1	293-301 bp	1.0	79.8