Hotspot

psbA-trnH

LSC · 82222-82565 · 344 bp

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Pi 0.0282

MarkerSeek score 63.80

Primer available yes

Label psbA-trnH

Feature Metadata

label_name: psbA-trnH
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 13
pi: 0.0282
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnH_p1	TTCCCTCTAGACCTAGCTGC	GATCCACTTGGCTACATCCG	55.0	55.0	57.9	58.1	322-414 bp	1.0	85.2
psbA-trnH_p2	TTCCCTCTAGACCTAGCTGC	CAATCCACTGCCTTGATCCA	55.0	50.0	57.9	57.9	336-428 bp	1.0	85.0
psbA-trnH_p3	TTCCCTCTAGACCTAGCTGC	AATCCACTGCCTTGATCCAC	55.0	50.0	57.9	57.9	335-427 bp	1.0	85.0
psbA-trnH_p4	TTCCCTCTAGACCTAGCTGC	ACAATCCACTGCCTTGATCC	55.0	50.0	57.9	57.9	337-429 bp	1.0	85.0
psbA-trnH_p5	TTCCCTCTAGACCTAGCTGC	ATTCATTTCTGGGCGAACGA	55.0	45.0	57.9	57.9	386-478 bp	1.0	84.9