Hotspot

rbcL-trnK

LSC · 77328-78180 · 853 bp

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Pi 0.0166

MarkerSeek score 62.60

Primer available yes

Label rbcL-trnK

Feature Metadata

label_name: rbcL-trnK
feature_type: igs
region: LSC
length_bp: 853
variable_sites: 21
pi: 0.0166
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-trnK_p1	AGTCCTGAATTAGCTGCTGC	AAAGCCGAGTACTCTACCGT	50.0	50.0	58.0	58.2	897-972 bp	1.0	84.1
rbcL-trnK_p2	AGTCCTGAATTAGCTGCTGC	AAGCCGAGTACTCTACCGTT	50.0	50.0	58.0	58.2	896-971 bp	1.0	84.1
rbcL-trnK_p3	GTGAGGCTTCCAAATGGAGT	AAGCCGAGTACTCTACCGTT	50.0	50.0	57.8	58.2	913-988 bp	1.0	82.8
rbcL-trnK_p4	GTGAGGCTTCCAAATGGAGT	AAAGCCGAGTACTCTACCGT	50.0	50.0	57.8	58.2	914-989 bp	1.0	82.7
rbcL-trnK_p5	GAGGCTTCCAAATGGAGTCC	AAGCCGAGTACTCTACCGTT	55.0	50.0	58.2	58.2	911-986 bp	1.0	82.5