Hotspot

ndhC-trnV

LSC · 71698-72152 · 455 bp

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Pi 0.0224

MarkerSeek score 62.90

Primer available yes

Label ndhC-trnV

Feature Metadata

label_name: ndhC-trnV
feature_type: igs
region: LSC
length_bp: 455
variable_sites: 15
pi: 0.0224
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV_p1	TCATATTCGTAAACCAAAAACA	AAAAGATGCACGTTGGGTCT	27.3	45.0	52.3	58.0	548-582 bp	1.0	46.1
ndhC-trnV_p2	TCATATTCGTAAACCAAAAACA	AAAGATGCACGTTGGGTCTT	27.3	45.0	52.3	58.0	547-581 bp	1.0	46.1
ndhC-trnV_p3	TCATATTCGTAAACCAAAAACA	AAGATGCACGTTGGGTCTTT	27.3	45.0	52.3	58.0	546-580 bp	1.0	46.1
ndhC-trnV_p4	TCATATTCGTAAACCAAAAACAT	AAAGATGCACGTTGGGTCTT	26.1	45.0	53.0	58.0	547-581 bp	1.0	46.1
ndhC-trnV_p5	TCATATTCGTAAACCAAAAACAT	AAGATGCACGTTGGGTCTTT	26.1	45.0	53.0	58.0	546-580 bp	1.0	46.1