Hotspot

trnT-trnL

LSC · 67702-68376 · 675 bp

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Pi 0.0174

MarkerSeek score 60.80

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 675
variable_sites: 17
pi: 0.0174
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	CGAAGCCATACCCAATCGAA	CCAACGCGAAAGGAAATGTC	50.0	50.0	58.1	58.0	1544-1576 bp	1.0	83.3
trnT-trnL_p2	AAAACCTCACCTGTTGACCC	TAACCCAACGCGAAAGGAAA	50.0	45.0	57.9	58.0	1490-1522 bp	1.0	83.3
trnT-trnL_p3	AAAACCTCACCTGTTGACCC	TTTCCTAACCCAACGCGAAA	50.0	45.0	57.9	58.0	1495-1527 bp	1.0	83.3
trnT-trnL_p4	CGAAGCCATACCCAATCGAA	TAACCCAACGCGAAAGGAAA	50.0	45.0	58.1	58.0	1548-1580 bp	1.0	83.2
trnT-trnL_p5	CGAAGCCATACCCAATCGAA	TTTCCTAACCCAACGCGAAA	50.0	45.0	58.1	58.0	1553-1585 bp	1.0	83.2