Hotspot

rps4-trnT

LSC · 67359-67628 · 270 bp

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Pi 0.037

MarkerSeek score 66.90

Primer available yes

Label rps4-trnT

Feature Metadata

label_name: rps4-trnT
feature_type: igs
region: LSC
length_bp: 270
variable_sites: 14
pi: 0.037
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT_p1	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	359-360 bp	1.0	80.1
rps4-trnT_p2	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	381-382 bp	1.0	80.0
rps4-trnT_p3	GAAACGAGGCCCTCTGTAAC	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	327-328 bp	1.0	79.5
rps4-trnT_p4	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	364-365 bp	1.0	79.4
rps4-trnT_p5	CTCCCAGACGGCGTATTTTT	ATGGTCATCGGTTCGATTCC	50.0	50.0	58.3	57.8	349-350 bp	1.0	79.4