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rpoC2-rps2

LSC · 57596-57799 · 204 bp

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Pi 0.0196

MarkerSeek score 66.10

Primer available yes

Label rpoC2-rps2

Feature Metadata

label_name: rpoC2-rps2
feature_type: igs
region: LSC
length_bp: 204
variable_sites: 5
pi: 0.0196
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-rps2_p1	TGTTCCACCACAGAGAAGTT	AATGAACTCCCGCTTCCATC	45.0	50.0	56.6	58.0	324-327 bp	1.0	70.9
rpoC2-rps2_p2	TTGTTCCACCACAGAGAAGT	AATGAACTCCCGCTTCCATC	45.0	50.0	56.6	58.0	325-328 bp	1.0	70.9
rpoC2-rps2_p3	TGTTCCACCACAGAGAAGTT	CCCGCTTCCATCATCTCTTC	45.0	55.0	56.6	58.1	316-319 bp	1.0	70.4
rpoC2-rps2_p4	TGTTCCACCACAGAGAAGTT	CCGCTTCCATCATCTCTTCC	45.0	55.0	56.6	58.1	315-318 bp	1.0	70.4
rpoC2-rps2_p5	TTGTTCCACCACAGAGAAGT	CCGCTTCCATCATCTCTTCC	45.0	55.0	56.6	58.1	316-319 bp	1.0	70.4