Hotspot

trnY-trnD

LSC · 43962-44375 · 414 bp

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Pi 0.0242

MarkerSeek score 64.50

Primer available yes

Label trnY-trnD

Feature Metadata

label_name: trnY-trnD
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 15
pi: 0.0242
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-trnD_p1	TGGCAATATGTCTACGCTGG	TATCTATCGGGACTGACGGG	50.0	55.0	57.8	57.8	525-535 bp	1.0	80.3
trnY-trnD_p2	GGCAATATGTCTACGCTGGT	TATCTATCGGGACTGACGGG	50.0	55.0	57.8	57.8	524-534 bp	1.0	80.3
trnY-trnD_p3	GGGGACGGACTGTAAATTCG	TATCTATCGGGACTGACGGG	55.0	55.0	58.4	57.8	546-556 bp	1.0	79.2
trnY-trnD_p4	GGGACGGACTGTAAATTCGT	TATCTATCGGGACTGACGGG	50.0	55.0	57.6	57.8	545-555 bp	1.0	79.1
trnY-trnD_p5	TGGCAATATGTCTACGCTGG	CGGTGCTCTGACCAATTGAA	50.0	50.0	57.8	58.5	474-484 bp	1.0	78.4