Hotspot

rps12-rpl20

LSC · 14818-15558 · 741 bp

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Pi 0.0063

MarkerSeek score 62.80

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: LSC
length_bp: 741
variable_sites: 7
pi: 0.0063
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	ACAGCCAATCCGAAATGTCA	TTTTGTCCTCCGTTTCCGAG	45.0	50.0	57.8	58.1	868-893 bp	1.0	78.0
rps12-rpl20_p2	ACAAGACAGCCAATCCGAAA	TTTTGTCCTCCGTTTCCGAG	45.0	50.0	57.7	58.1	873-898 bp	1.0	77.5
rps12-rpl20_p3	AGGAACGTGTACTAGGGTGT	TTTTGTCCTCCGTTTCCGAG	50.0	50.0	57.7	58.1	811-836 bp	1.0	77.3
rps12-rpl20_p4	ACAGCCAATCCGAAATGTCA	TTTTTGTCCTCCGTTTCCGA	45.0	45.0	57.8	57.7	869-894 bp	1.0	76.4
rps12-rpl20_p5	ACAAGACAGCCAATCCGAAA	TTTTTGTCCTCCGTTTCCGA	45.0	45.0	57.7	57.7	874-899 bp	1.0	75.8