Hotspot

psbB-clpP

LSC · 12053-12523 · 471 bp

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Pi 0.0297

MarkerSeek score 71.80

Primer available yes

Label psbB-clpP

Feature Metadata

label_name: psbB-clpP
feature_type: igs
region: LSC
length_bp: 471
variable_sites: 21
pi: 0.0297
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB-clpP_p1	AAACGGCCGGGATCATTTAA	CTTTTGGAACACCAATGGGC	45.0	50.0	57.9	57.8	548-549 bp	1.0	80.8
psbB-clpP_p2	AACGGCCGGGATCATTTAAA	CTTTTGGAACACCAATGGGC	45.0	50.0	57.9	57.8	547-548 bp	1.0	80.8
psbB-clpP_p3	ACGGCCGGGATCATTTAAAA	CTTTTGGAACACCAATGGGC	45.0	50.0	57.9	57.8	546-547 bp	1.0	80.8
psbB-clpP_p4	AACGGCCGGGATCATTTAAA	TTTGGAACACCAATGGGCAT	45.0	45.0	57.9	58.3	545-546 bp	1.0	80.0
psbB-clpP_p5	ACGGCCGGGATCATTTAAAA	TTTGGAACACCAATGGGCAT	45.0	45.0	57.9	58.3	544-545 bp	1.0	80.0