Hotspot

ndhI-ndhA

SSC · 120552-120649 · 98 bp

Back to Gelsemium

Pi 0.0306

MarkerSeek score 65.50

Primer available yes

Label ndhI-ndhA

Feature Metadata

label_name: ndhI-ndhA
feature_type: igs
region: SSC
length_bp: 98
variable_sites: 3
pi: 0.0306
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI-ndhA_p1	AACCGATTCGTGTGAGACAG	CAAGGCTGAGAATGGACCAA	50.0	50.0	57.9	57.8	312 bp	1.0	78.6
ndhI-ndhA_p2	AACCGATTCGTGTGAGACAG	AAGGCTGAGAATGGACCAAC	50.0	50.0	57.9	57.8	311 bp	1.0	78.6
ndhI-ndhA_p3	TGGCAGCTCGTATTGTTTGT	CAAGGCTGAGAATGGACCAA	45.0	50.0	58.1	57.8	263 bp	1.0	78.4
ndhI-ndhA_p4	TGGCAGCTCGTATTGTTTGT	AAGGCTGAGAATGGACCAAC	45.0	50.0	58.1	57.8	262 bp	1.0	78.4
ndhI-ndhA_p5	TGAAACCTTGGCCGATGTAC	CAAGGCTGAGAATGGACCAA	50.0	50.0	58.2	57.8	284 bp	1.0	77.7