Hotspot

ndhF-rpl32

SSC · 113477-114243 · 767 bp

Back to Gelsemium

Pi 0.0248

MarkerSeek score 67.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 767
variable_sites: 19
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGCCGTGGGAAAAAGAAGAA	CGCTGTCCAATATCCCTTCC	45.0	55.0	57.9	58.1	946-947 bp	1.0	80.4
ndhF-rpl32_p2	TTGCCGTGGGAAAAAGAAGA	CGCTGTCCAATATCCCTTCC	45.0	55.0	57.9	58.1	947-948 bp	1.0	80.4
ndhF-rpl32_p3	GTTGCCGTGGGAAAAAGAAG	CGCTGTCCAATATCCCTTCC	50.0	55.0	57.9	58.1	948-949 bp	1.0	79.8
ndhF-rpl32_p4	TTTGTTGCCGTGGGAAAAAG	CGCTGTCCAATATCCCTTCC	45.0	55.0	57.7	58.1	951-952 bp	1.0	78.4
ndhF-rpl32_p5	TGTTGCCGTGGGAAAAAGAA	CGCTGTCCAATATCCCTTCC	45.0	55.0	58.5	58.1	949-950 bp	1.0	76.6