Hotspot

petD-rps11

LSC · 79182-80446 · 1265 bp

Back to Gelsemium

Pi 0.00632

MarkerSeek score 62.40

Primer available yes

Label petD-rps11

Feature Metadata

label_name: petD-rps11
feature_type: igs
region: LSC
length_bp: 1265
variable_sites: 8
pi: 0.00632
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rps11_p1	TTGGTTGGGTATTGGTGCAA	ACTCCGGGCTATTCGTAGAA	45.0	50.0	57.9	57.9	1429-1433 bp	1.0	79.2
petD-rps11_p2	TTGGTTGGGTATTGGTGCAA	TCCGGGCTATTCGTAGAAGT	45.0	50.0	57.9	57.9	1427-1431 bp	1.0	79.2
petD-rps11_p3	TTTGGTTGGGTATTGGTGCA	ACTCCGGGCTATTCGTAGAA	45.0	50.0	57.9	57.9	1430-1434 bp	1.0	79.2
petD-rps11_p4	TTTGGTTGGGTATTGGTGCA	TCCGGGCTATTCGTAGAAGT	45.0	50.0	57.9	57.9	1428-1432 bp	1.0	79.2
petD-rps11_p5	CGTCGTCCTGTAGCTACAAC	TCCGGGCTATTCGTAGAAGT	55.0	50.0	58.1	57.9	1477-1481 bp	1.0	79.1