Hotspot

psbE-petL

LSC · 66270-67283 · 1014 bp

Back to Gelsemium

Pi 0.0684

MarkerSeek score 90.70

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1014
variable_sites: 67
pi: 0.0684
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 90.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	57.9	57.9	1178-1185 bp	1.0	69.8
psbE-petL_p2	AGAACGTTCTCCTGTGCTTC	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	57.8	57.9	1101-1108 bp	1.0	69.4
psbE-petL_p3	ACGAATAACCAACCCGCAAT	AGCGGAGGTTATAGTTAAAGCC	45.0	45.5	58.2	57.9	1187-1194 bp	1.0	68.8
psbE-petL_p4	CAACCCGCAATGAATAGGGA	GCGGAGGTTATAGTTAAAGCCA	50.0	45.5	57.9	58.2	1177-1184 bp	1.0	68.8
psbE-petL_p5	TGCTGACGAATAACCAACCC	AGCGGAGGTTATAGTTAAAGCC	50.0	45.5	58.2	57.9	1192-1199 bp	1.0	68.7