Hotspot

psbL-psbF

LSC · 65866-65888 · 23 bp

Back to Gelsemium

Pi 0.087

MarkerSeek score 82.00

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 23
variable_sites: 2
pi: 0.087
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCGTTCGGGTTTGATTGTGT	AGTACGATGGTTGGCTGTTC	45.0	50.0	58.0	57.9	134 bp	1.0	80.1
psbL-psbF_p2	TCGTTCGGGTTTGATTGTGT	CAGTACGATGGTTGGCTGTT	45.0	50.0	58.0	58.2	135 bp	1.0	79.3
psbL-psbF_p3	TCGTTCGGGTTTGATTGTGT	GTACGATGGTTGGCTGTTCA	45.0	50.0	58.0	58.2	133 bp	1.0	79.3
psbL-psbF_p4	TCGTTCGGGTTTGATTGTGT	TACGATGGTTGGCTGTTCAC	45.0	50.0	58.0	58.2	132 bp	1.0	79.3
psbL-psbF_p5	TCGTTCGGGTTTGATTGTGT	CCTAGCTGTACCTACCGTCT	45.0	55.0	58.0	57.7	110 bp	1.0	78.3