Hotspot

ndhC-trnV-UAC

LSC · 51869-53028 · 1160 bp

Back to Gelsemium

Pi 0.038
MarkerSeek score 71.50
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1160
variable_sites
44
pi
0.038
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TCATATTCGTAAAGCAGAAACA AGAAGGTCTACGGTTCGAGT 31.8 50.0 53.9 57.8 1216-1225 bp 1.0 43.0
ndhC-trnV-UAC_p2 AGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 33.3 50.0 56.1 57.8 1265-1274 bp 1.0 42.9
ndhC-trnV-UAC_p3 AAGCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 32.0 50.0 56.7 57.8 1266-1275 bp 1.0 42.9
ndhC-trnV-UAC_p4 AGCCAAAATAGGAATAAGACTTGA GAAGGTCTACGGTTCGAGTC 33.3 55.0 56.1 57.5 1264-1273 bp 1.0 42.9
ndhC-trnV-UAC_p5 GCCAAAATAGGAATAAGACTTGA AGAAGGTCTACGGTTCGAGT 34.8 50.0 54.7 57.8 1264-1273 bp 1.0 42.9