Hotspot

atpF-atpH

LSC · 13443-13794 · 352 bp

Back to Gelsemium

Pi 0.0483

MarkerSeek score 81.40

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 352
variable_sites: 17
pi: 0.0483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TATTAACCCCGAAACTCCCG	AGGCAGAGGGAAAAATACGG	50.0	50.0	57.0	57.6	538-539 bp	1.0	71.3
atpF-atpH_p2	GGTATTAACCCCGAAACTCCC	AGGCAGAGGGAAAAATACGG	52.4	50.0	58.1	57.6	540-541 bp	1.0	70.5
atpF-atpH_p3	TATTAACCCCGAAACTCCCG	GCAGAGGGAAAAATACGGGG	50.0	55.0	57.0	58.6	536-537 bp	1.0	69.8
atpF-atpH_p4	TATTAACCCCGAAACTCCCG	GGCAGAGGGAAAAATACGGG	50.0	55.0	57.0	58.6	537-538 bp	1.0	69.8
atpF-atpH_p5	GGTATTAACCCCGAAACTCCC	GCAGAGGGAAAAATACGGGG	52.4	55.0	58.1	58.6	538-539 bp	1.0	69.0