Hotspot

rpoA

LSC · 76896-77915 · 1020 bp

Back to Gelidocalamus

Pi 0.00219

MarkerSeek score 36.50

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: LSC
length_bp: 1020
variable_sites: 12
pi: 0.00219
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CGAGTGGTACTTCTTTCCCG	TAAAGGGTGCTGGTAGTGGA	55.0	50.0	58.0	58.0	1684-1690 bp	1.0	81.0
rpoA_p2	CGAGTGGTACTTCTTTCCCG	TTCGTACAGTGGGTTTGCAA	55.0	45.0	58.0	58.0	1720-1726 bp	1.0	80.9
rpoA_p3	CGAGTGGTACTTCTTTCCCG	GTGGAAGAGATGCCGCATTA	55.0	50.0	58.0	58.0	1669-1675 bp	1.0	80.9
rpoA_p4	CGAGTGGTACTTCTTTCCCG	TGGAAGAGATGCCGCATTAC	55.0	50.0	58.0	58.0	1668-1674 bp	1.0	80.9
rpoA_p5	CGAGTGGTACTTCTTTCCCG	TCACGTAGGAGAGTGCGTAT	55.0	50.0	58.0	58.0	1955-1961 bp	1.0	80.8