Hotspot

rpl22

LSC · 82711-83160 · 450 bp

Back to Gelidocalamus

Pi 0.00422

MarkerSeek score 30.10

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: LSC
length_bp: 450
variable_sites: 6
pi: 0.00422
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	GTGCGAACCAAAAGGAATGG	CAAATTGGGGGAATTCGTGC	50.0	50.0	57.9	58.0	721-737 bp	1.0	84.5
rpl22_p2	GTGCGAACCAAAAGGAATGG	GCAAATTGGGGGAATTCGTG	50.0	50.0	57.9	58.0	722-738 bp	1.0	84.5
rpl22_p3	CAGAACTTGAGCCTGAGTCC	CAAATTGGGGGAATTCGTGC	55.0	50.0	57.9	58.0	889-905 bp	1.0	83.5
rpl22_p4	CAGAACTTGAGCCTGAGTCC	GCAAATTGGGGGAATTCGTG	55.0	50.0	57.9	58.0	890-906 bp	1.0	83.5
rpl22_p5	GTGCGAACCAAAAGGAATGG	GTTATCCTGCGCTTGGAAGA	50.0	50.0	57.9	58.0	1262-1278 bp	1.0	82.2