Hotspot

rpl32-trnL-UAG

SSC · 108392-109093 · 702 bp

Back to Gelidocalamus

Pi 0.00672

MarkerSeek score 43.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 702
variable_sites: 15
pi: 0.00672
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AAGGGTTTTTCTGGGCAACA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	774-797 bp	1.0	79.7
rpl32-trnL-UAG_p2	AGGGTTTTTCTGGGCAACAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.1	57.9	773-796 bp	1.0	79.7
rpl32-trnL-UAG_p3	AAGGGTTTTTCTGGGCAACA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	767-790 bp	1.0	79.3
rpl32-trnL-UAG_p4	AGGGTTTTTCTGGGCAACAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.1	57.8	766-789 bp	1.0	79.3
rpl32-trnL-UAG_p5	AGGGTTTTTCTGGGCAACAA	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.1	58.2	787-810 bp	1.0	79.3