Hotspot

rpl22-rps19

IRb · 83161-83237 · 77 bp

Back to Gelidocalamus

Pi 0.0637

MarkerSeek score 65.80

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 77
variable_sites: 12
pi: 0.0637
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	ACCAGTTTGAAACTTGTCAT	CAAATTGGGGGAATTCGTGC	35.0	50.0	53.2	58.0	162-177 bp	1.0	51.2
rpl22-rps19_p2	ACCAGTTTGAAACTTGTCAT	GCAAATTGGGGGAATTCGTG	35.0	50.0	53.2	58.0	163-178 bp	1.0	51.2
rpl22-rps19_p3	ACTTTACCAGTTTGAAACTTGTCA	CAAATTGGGGGAATTCGTGC	33.3	50.0	57.2	58.0	167-182 bp	1.0	50.8
rpl22-rps19_p4	ACTTTACCAGTTTGAAACTTGTCA	GCAAATTGGGGGAATTCGTG	33.3	50.0	57.2	58.0	168-183 bp	1.0	50.8
rpl22-rps19_p5	ACCAGTTTGAAACTTGTCAT	ATGGTAGGTCGCAAATTGGG	35.0	50.0	53.2	58.2	173-188 bp	1.0	49.3