Hotspot

ndhC-trnV-UAC

LSC · 52460-53215 · 756 bp

Back to Gelidocalamus

Pi 0.00305

MarkerSeek score 52.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 10
pi: 0.00305
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAATCGGGGCTA	GGTTCGAATCCGTATAGCCC	50.0	55.0	57.5	57.9	877-889 bp	1.0	78.3
ndhC-trnV-UAC_p2	CTGGTCCTTCACTAATCGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	57.4	57.9	881-893 bp	1.0	77.2
ndhC-trnV-UAC_p3	TCCTTCACTAATCGGGGCTA	CGGTTCGAATCCGTATAGCC	50.0	55.0	57.5	58.3	878-890 bp	1.0	76.5
ndhC-trnV-UAC_p4	TGGTCCTTCACTAATCGGGG	GGTTCGAATCCGTATAGCCC	55.0	55.0	58.8	57.9	880-892 bp	1.0	75.6
ndhC-trnV-UAC_p5	CTGGTCCTTCACTAATCGGG	CGGTTCGAATCCGTATAGCC	55.0	55.0	57.4	58.3	882-894 bp	1.0	75.3