Hotspot

trnF-GAA-ndhJ

LSC · 50205-50787 · 583 bp

Back to Gelidocalamus

Pi 0.00573

MarkerSeek score 51.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 583
variable_sites: 12
pi: 0.00573
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	691-697 bp	1.0	80.2
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	681-687 bp	1.0	79.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	ATCATCCACGCCTTAAACGT	50.0	45.0	58.1	57.6	722-728 bp	1.0	78.1
trnF-GAA-ndhJ_p4	AATCCTCGTGTCACCAGTTC	TGAAAGTTGGATAGGCTGGC	50.0	50.0	57.5	57.9	694-700 bp	1.0	76.9
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGCTGGCC	50.0	55.0	58.1	58.6	690-696 bp	1.0	76.4