Hotspot

trnG-UCC-trnfM-CAU

LSC · 12974-13414 · 441 bp

Back to Gelidocalamus

Pi 0.00504

MarkerSeek score 42.40

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 441
variable_sites: 10
pi: 0.00504
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	GCGAGCGTAGTTCAATGGTA	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.1	57.8	559-574 bp	1.0	79.1
trnG-UCC-trnfM-CAU_p2	GCGAGCGTAGTTCAATGGTA	CAGTTTGGTAGCTCACGAGG	50.0	55.0	58.1	58.3	574-589 bp	1.0	78.6
trnG-UCC-trnfM-CAU_p3	CGTAGCGAGCGTAGTTCAAT	CGAGGCTCATAACCTTGAGG	50.0	55.0	58.2	57.8	563-578 bp	1.0	78.3
trnG-UCC-trnfM-CAU_p4	TAGCGAGCGTAGTTCAATGG	CGAGGCTCATAACCTTGAGG	50.0	55.0	57.8	57.8	561-576 bp	1.0	78.1
trnG-UCC-trnfM-CAU_p5	GCGAGCGTAGTTCAATGGTA	GAGCGGAGTAGAGCAGTTTG	50.0	55.0	58.1	58.4	587-602 bp	1.0	77.9