Hotspot

ycf37

Genome · 100196-100648 · 453 bp

Back to Gelidium

Pi 0.0618

MarkerSeek score 75.80

Primer available yes

Label ycf37

Feature Metadata

label_name: ycf37
feature_type: gene
region: Genome
length_bp: 453
variable_sites: 50
pi: 0.0618
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf37_p1	CGAGGCACCTGTATTTCTGG	TGTTGGTTGGGTTAGAAGGC	55.0	50.0	58.3	58.0	1635-1636 bp	1.0	82.8
ycf37_p2	AGAACCCTTTGGCCTTGTTT	TGTTGGTTGGGTTAGAAGGC	45.0	50.0	57.8	58.0	1145 bp	0.75	72.0
ycf37_p3	AGAACCCTTTGGCCTTGTTT	TCGGGATATAGCGCAGTTTG	45.0	50.0	57.8	57.9	744 bp	0.75	71.8
ycf37_p4	GAACCCTTTGGCCTTGTTTG	TGTTGGTTGGGTTAGAAGGC	50.0	50.0	57.8	58.0	1144 bp	0.5	58.2
ycf37_p5	GAACCCTTTGGCCTTGTTTG	TCGGGATATAGCGCAGTTTG	50.0	50.0	57.8	57.9	743 bp	0.5	57.2