Hotspot

infB

Genome · 30859-33060 · 2202 bp

Back to Gelidium

Pi 0.0441

MarkerSeek score 72.20

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2202
variable_sites: 176
pi: 0.0441
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	CATGCCATCCCGGTAGTAAC	TATCACGTCGCTCTACTGGT	55.0	50.0	58.1	58.0	2638 bp	1.0	84.6
infB_p2	CATGCCATCCCGGTAGTAAC	ACGTCGCTCTACTGGTCTTA	55.0	50.0	58.1	57.9	2634 bp	1.0	84.1
infB_p3	CATGCCATCCCGGTAGTAAC	GGGAAAAACAAAGGAGCACG	55.0	50.0	58.1	57.9	2949 bp	1.0	83.6
infB_p4	CATGCCATCCCGGTAGTAAC	TGGGGAAAAACAAAGGAGCA	55.0	45.0	58.1	57.8	2951 bp	1.0	83.3
infB_p5	CATGCCATCCCGGTAGTAAC	GGAATACACCACAGCGCTTA	55.0	50.0	58.1	58.0	2834 bp	0.5	58.9