Hotspot

ccdA

Genome · 116775-117494 · 720 bp

Back to Gelidium

Pi 0.0407

MarkerSeek score 71.10

Primer available yes

Label ccdA

Feature Metadata

label_name: ccdA
feature_type: gene
region: Genome
length_bp: 720
variable_sites: 52
pi: 0.0407
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccdA_p1	AGCTGCGAACAACGATACAT	GGCGCAATTCTTCCAAACAA	45.0	45.0	58.0	57.9	1878 bp	0.75	71.9
ccdA_p2	GGTTCAAATCCTTCCTGGGG	GGCGCAATTCTTCCAAACAA	55.0	45.0	58.1	57.9	1309 bp	0.75	70.9
ccdA_p3	AGCTGCGAACAACGATACAT	CGAAAGAGTGCAGCCAGTTA	45.0	50.0	58.0	58.2	1675 bp	0.5	58.4
ccdA_p4	CCACGTCCGCTTTAAAGGTA	GGCGCAATTCTTCCAAACAA	50.0	45.0	57.9	57.9	1495 bp	0.5	58.3
ccdA_p5	CCACGTCCGCTTTAAAGGTA	CGAAAGAGTGCAGCCAGTTA	50.0	50.0	57.9	58.2	1292 bp	0.5	57.8