Hotspot

ORF614

Genome · 54552-56471 · 1920 bp

Back to Gelidium

Pi 0.0523

MarkerSeek score 68.40

Primer available yes

Label ORF614

Feature Metadata

label_name: ORF614
feature_type: gene
region: Genome
length_bp: 1920
variable_sites: 184
pi: 0.0523
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF614_p1	TGACCCATCAAGAAATCCTAACA	ACAAGGGCCGAATAGTAAGC	39.1	50.0	57.5	57.7	2773-2788 bp	0.75	42.8
ORF614_p2	TGACCCATCAAGAAATCCTAACA	CAAAACGTTGCAGTTCAGCA	39.1	45.0	57.5	58.1	2861 bp	0.5	31.2
ORF614_p3	TGACCCATCAAGAAATCCTAACA	TCAAAACGTTGCAGTTCAGC	39.1	45.0	57.5	57.8	2862 bp	0.5	30.4
ORF614_p4	TCAGGAACATAAGGATGCTTACA	CAAAACGTTGCAGTTCAGCA	39.1	45.0	57.3	58.1	2952 bp	0.5	29.7
ORF614_p5	ACCCATCAAGAAATCCTAACAGA	CAAAACGTTGCAGTTCAGCA	39.1	45.0	57.2	58.1	2859 bp	0.5	29.0