Hotspot

ORF12

Genome · 58048-59241 · 1194 bp

Back to Gelidium

Pi 0.0565

MarkerSeek score 79.70

Primer available yes

Label ORF12

Feature Metadata

label_name: ORF12
feature_type: gene
region: Genome
length_bp: 1194
variable_sites: 121
pi: 0.0565
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ORF12_p1	TATAGCATCTGTGGCCGAGA	CACGTGTGGGTCAACCTTTA	50.0	50.0	58.0	58.0	2136-2139 bp	0.75	74.4
ORF12_p2	TCTATAGCATCTGTGGCCGA	CACGTGTGGGTCAACCTTTA	50.0	50.0	58.0	58.0	2138-2141 bp	0.75	74.4
ORF12_p3	TATAGCATCTGTGGCCGAGA	ACTAACACGTGTGGGTCAAC	50.0	50.0	58.0	58.1	2141-2144 bp	0.75	74.3
ORF12_p4	TCTATAGCATCTGTGGCCGA	ACTAACACGTGTGGGTCAAC	50.0	50.0	58.0	58.1	2143-2146 bp	0.75	74.3
ORF12_p5	TATAGCATCTGTGGCCGAGA	CACTAACACGTGTGGGTCAA	50.0	50.0	58.0	58.1	2142-2145 bp	0.75	74.3