Hotspot

trnY-trnT

Genome · 152660-152680 · 21 bp

Back to Gelidium

Pi 0.0476

MarkerSeek score 72.10

Primer available yes

Label trnY-trnT

Feature Metadata

label_name: trnY-trnT
feature_type: igs
region: Genome
length_bp: 21
variable_sites: 2
pi: 0.0476
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnY-trnT_p1	ACTGTAAATCCGCTGGCTTT	CTTCCACTGAGCTATAAGGGC	45.0	52.4	57.8	57.9	89-93 bp	1.0	74.7
trnY-trnT_p2	GACTGTAAATCCGCTGGCTT	CTTCCACTGAGCTATAAGGGC	50.0	52.4	58.3	57.9	90-94 bp	1.0	74.1
trnY-trnT_p3	ACTGTAAATCCGCTGGCTTT	TTCCACTGAGCTATAAGGGC	45.0	50.0	57.8	56.7	88-92 bp	1.0	73.3
trnY-trnT_p4	GACTGTAAATCCGCTGGCTT	TTCCACTGAGCTATAAGGGC	50.0	50.0	58.3	56.7	89-93 bp	1.0	72.7
trnY-trnT_p5	CTGTAAATCCGCTGGCTTTG	CTTCCACTGAGCTATAAGGGC	50.0	52.4	57.5	57.9	88-92 bp	1.0	71.9