Hotspot

trnI-rrs

Genome · 125156-125221 · 66 bp

Back to Gelidium

Pi 0.0581

MarkerSeek score 72.80

Primer available yes

Label trnI-rrs

Feature Metadata

label_name: trnI-rrs
feature_type: igs
region: Genome
length_bp: 66
variable_sites: 7
pi: 0.0581
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-rrs_p1	TTTTCACCTTTCCCTCACGG	TAGTAATCGCCGGTCAGCTA	50.0	50.0	58.0	58.0	968 bp	1.0	81.9
trnI-rrs_p2	TTTTCACCTTTCCCTCACGG	CCTTACGTTCTGGGCTACAC	50.0	55.0	58.0	58.0	1104 bp	1.0	81.8
trnI-rrs_p3	TTTTCACCTTTCCCTCACGG	CTAAGTTCGGATTGCAGGCT	50.0	50.0	58.0	58.0	1017 bp	1.0	81.8
trnI-rrs_p4	TTTTCACCTTTCCCTCACGG	CTAGATGTTGCGCGTATCGA	50.0	50.0	58.0	58.0	1487 bp	1.0	81.2
trnI-rrs_p5	TTTTCACCTTTCCCTCACGG	TCCGTGCAGTATCGTAGCTA	50.0	50.0	58.0	58.0	1467 bp	1.0	81.1