Hotspot

trpG-thiG

Genome · 119408-119518 · 111 bp

Back to Gelidium

Pi 0.048

MarkerSeek score 74.40

Primer available yes

Label trpG-thiG

Feature Metadata

label_name: trpG-thiG
feature_type: igs
region: Genome
length_bp: 111
variable_sites: 9
pi: 0.048
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trpG-thiG_p1	AGATGGCCATTTTGTGTCCA	TGCCAAATACAGCTGGTTGT	45.0	45.0	57.7	58.0	1236-1240 bp	0.75	71.1
trpG-thiG_p2	ACTTTTCCATGCACAGGCTT	GGTGTATTGTCTGGACGCAT	45.0	50.0	58.3	58.0	520-524 bp	0.75	70.7
trpG-thiG_p3	ACTTTTCCATGCACAGGCTT	GTGAAGCAAGCAGAGCAATG	45.0	50.0	58.3	58.0	624-628 bp	0.75	70.6
trpG-thiG_p4	ACTTTTCCATGCACAGGCTT	TGCCAAATACAGCTGGTTGT	45.0	45.0	58.3	58.0	1008-1012 bp	0.75	70.3
trpG-thiG_p5	TTCCTGCTTTCTGTGGTGTG	TGCCAAATACAGCTGGTTGT	50.0	45.0	58.3	58.0	878-882 bp	0.75	70.2