Hotspot

trnS-psaD

Genome · 11129-13431 · 2303 bp

Back to Gelidium

Pi 0.0829

MarkerSeek score 63.50

Primer available yes

Label trnS-psaD

Feature Metadata

label_name: trnS-psaD
feature_type: igs
region: Genome
length_bp: 2303
variable_sites: 288
pi: 0.0829
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.66
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-psaD_p1	ACCAACTCTACCACCTCTCC	AGCAAGAAAAGAGCAGTGCT	55.0	45.0	58.1	58.0	343-2564 bp	1.0	91.3
trnS-psaD_p2	ACCAACTCTACCACCTCTCC	GGTAGTACAGGGGGATGGTT	55.0	55.0	58.1	58.1	483-2704 bp	1.0	90.6
trnS-psaD_p3	ACCAACTCTACCACCTCTCC	CCGATAGAATCAGCAAGCGT	55.0	50.0	58.1	58.1	719-2979 bp	1.0	90.4
trnS-psaD_p4	ATACCTTACGGAGAGGGTGG	AGCAAGAAAAGAGCAGTGCT	55.0	45.0	57.9	58.0	1891-2638 bp	0.75	81.2
trnS-psaD_p5	ATACCTTACGGAGAGGGTGG	GGTAGTACAGGGGGATGGTT	55.0	55.0	57.9	58.1	2031-2778 bp	0.75	80.4