Hotspot

trnR-ACG

IRa · 129181-129254 · 74 bp

Back to Gelasia

Pi 0.0135
MarkerSeek score 60.30
Primer available yes
Label trnR-ACG

Feature Metadata

label_name
trnR-ACG
feature_type
tRNA
region
IRa
length_bp
74
variable_sites
1
pi
0.0135
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG_p1 TCTTAACCCGAAATGGCTGG TCCGCTATCCCGATCATGAT 50.0 50.0 57.9 58.1 212 bp 1.0 78.2
trnR-ACG_p2 TCTTAACCCGAAATGGCTGG AAGGCCATTCCATTTCGACA 50.0 45.0 57.9 57.8 264 bp 1.0 77.6
trnR-ACG_p3 TCTTAACCCGAAATGGCTGG AGGCCATTCCATTTCGACAA 50.0 45.0 57.9 57.8 263 bp 1.0 77.6
trnR-ACG_p4 AAGGCGCTTTGACATCTTCT TCCGCTATCCCGATCATGAT 45.0 50.0 57.8 58.1 229 bp 1.0 77.6
trnR-ACG_p5 AGGCGCTTTGACATCTTCTT TCCGCTATCCCGATCATGAT 45.0 50.0 57.8 58.1 228 bp 1.0 77.6
trnR-ACG_p1 TCCGCTATCCCGATCATGAT TCTTAACCCGAAATGGCTGG 50.0 50.0 58.1 57.9 212 bp 1.0 78.2
trnR-ACG_p2 AAGGCCATTCCATTTCGACA TCTTAACCCGAAATGGCTGG 45.0 50.0 57.8 57.9 264 bp 1.0 77.6
trnR-ACG_p3 AGGCCATTCCATTTCGACAA TCTTAACCCGAAATGGCTGG 45.0 50.0 57.8 57.9 263 bp 1.0 77.6
trnR-ACG_p4 TCCGCTATCCCGATCATGAT AAGGCGCTTTGACATCTTCT 50.0 45.0 58.1 57.8 229 bp 1.0 77.6
trnR-ACG_p5 TCCGCTATCCCGATCATGAT AGGCGCTTTGACATCTTCTT 50.0 45.0 58.1 57.8 228 bp 1.0 77.6