Hotspot

petA

LSC · 62132-63094 · 963 bp

Back to Gelasia

Pi 0.00208

MarkerSeek score 59.90

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 963
variable_sites: 2
pi: 0.00208
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	TTCGGGATCAATGGTTGGAC	TGATGTCGATAAATCCGCGG	50.0	50.0	57.9	58.2	1006 bp	1.0	77.4
petA_p2	TTCGGGATCAATGGTTGGAC	GATGTCGATAAATCCGCGGG	50.0	55.0	57.9	58.9	1005 bp	1.0	71.7
petA_p3	TTTCGGGATCAATGGTTGGA	TGATGTCGATAAATCCGCGG	45.0	50.0	57.1	58.2	1007 bp	1.0	71.3
petA_p4	TCCATTAGTGACCTACCCCA	TGATGTCGATAAATCCGCGG	50.0	50.0	57.0	58.2	1042 bp	1.0	70.8
petA_p5	TTCGGGATCAATGGTTGGAC	ATGTCGATAAATCCGCGGG	50.0	52.6	57.9	57.8	1004 bp	1.0	69.5