Hotspot

ndhG

SSC · 117643-118173 · 531 bp

Back to Gelasia

Pi 0.00377

MarkerSeek score 54.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 2
pi: 0.00377
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	ACAAGGTTTTGTTCCAGTTCT	CTTTTTCCTGACCGGGTCAA	38.1	50.0	55.8	58.0	628-629 bp	1.0	54.8
ndhG_p2	GCATGTGATAGCTCATAACAAGG	CTTTTTCCTGACCGGGTCAA	43.5	50.0	57.6	58.0	645-646 bp	1.0	53.3
ndhG_p3	ACAAGGTTTTGTTCCAGTTCT	TTCTTTTTCCTGACCGGGTC	38.1	50.0	55.8	57.7	630-631 bp	1.0	52.8
ndhG_p4	ACAAGGTTTTGTTCCAGTTCT	ACTTCTTTTTCCTGACCGGG	38.1	50.0	55.8	57.7	632-633 bp	1.0	52.8
ndhG_p5	ACAAGGTTTTGTTCCAGTTCT	CTTCTTTTTCCTGACCGGGT	38.1	50.0	55.8	57.7	631-632 bp	1.0	52.8