Hotspot

atpH

LSC · 26200-26445 · 246 bp

Back to Gelasia

Pi 0.00407

MarkerSeek score 41.30

Primer available yes

Label atpH

Feature Metadata

label_name: atpH
feature_type: gene
region: LSC
length_bp: 246
variable_sites: 1
pi: 0.00407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH_p1	CGGCCCAATCCATTTATCCA	AAGTCCTACGAACACGAACG	50.0	50.0	58.0	58.0	1068 bp	1.0	79.9
atpH_p2	TTTCTATGCAAGGAGTCGGC	AAGTCCTACGAACACGAACG	50.0	50.0	58.0	58.0	1084 bp	1.0	79.8
atpH_p3	CGGCCCAATCCATTTATCCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.0	57.9	1288 bp	1.0	79.7
atpH_p4	TTTCTATGCAAGGAGTCGGC	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.0	57.9	1304 bp	1.0	79.6
atpH_p5	TAGAGGCGAGGTCGAACTAG	AAGTCCTACGAACACGAACG	55.0	50.0	58.0	58.0	961 bp	1.0	79.6