Hotspot

ndhF-rpl32

SSC · 111376-112486 · 1111 bp

Back to Gelasia

Pi 0.0045

MarkerSeek score 61.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1111
variable_sites: 5
pi: 0.0045
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	TTTAAGGCGGCCCAATATCC	50.0	50.0	58.0	58.0	1298 bp	1.0	80.0
ndhF-rpl32_p2	TGCCGTCGGAAAAAGAAGAA	TTTAAGGCGGCCCAATATCC	45.0	50.0	58.0	58.0	1296 bp	1.0	79.9
ndhF-rpl32_p3	TTGCCGTCGGAAAAAGAAGA	TTTAAGGCGGCCCAATATCC	45.0	50.0	58.0	58.0	1297 bp	1.0	79.9
ndhF-rpl32_p4	ACATAGGAACGGGAAGTGGA	TTTAAGGCGGCCCAATATCC	50.0	50.0	58.1	58.0	1261 bp	1.0	79.8
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	TTTAAGGCGGCCCAATATCC	45.0	50.0	57.8	58.0	1301 bp	1.0	78.7