Hotspot

pafII-cemA

LSC · 60328-61221 · 894 bp

Back to Gelasia

Pi 0.00225

MarkerSeek score 60.70

Primer available yes

Label pafII-cemA

Feature Metadata

label_name: pafII-cemA
feature_type: igs
region: LSC
length_bp: 894
variable_sites: 2
pi: 0.00225
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
pafII-cemA_p1	GAGCCATTCCATTGACTCGT	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.0	58.1	1074 bp	1.0	79.7
pafII-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGAAATCCACCAGGGCAAA	45.0	50.0	58.1	58.1	1006 bp	1.0	79.3
pafII-cemA_p3	AAAAAGCTGCTGAATTGGCC	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1020 bp	1.0	78.6
pafII-cemA_p4	AAAGCTGCTGAATTGGCCTA	GAGAAATCCACCAGGGCAAA	45.0	50.0	57.8	58.1	1018 bp	1.0	78.3
pafII-cemA_p5	CGGGAAATGGAGCAAAAAGC	GAGAAATCCACCAGGGCAAA	50.0	50.0	58.3	58.1	1033 bp	1.0	77.7