Hotspot

trnS-GCU-trnC-GCA

LSC · 8659-9426 · 768 bp

Back to Gelasia

Pi 0.0026

MarkerSeek score 53.30

Primer available yes

Label trnS-GCU-trnC-GCA

Feature Metadata

label_name: trnS-GCU-trnC-GCA
feature_type: igs
region: LSC
length_bp: 768
variable_sites: 2
pi: 0.0026
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnC-GCA_p1	ACGGAAAGAGAGGGATTCGA	GCTTCGTTTGTTGATGAGGC	50.0	50.0	58.2	58.0	944-950 bp	1.0	78.7
trnS-GCU-trnC-GCA_p2	ACGGAAAGAGAGGGATTCGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	919-925 bp	1.0	78.3
trnS-GCU-trnC-GCA_p3	ACGGAAAGAGAGGGATTCGA	CTTCGTTTGTTGATGAGGCG	50.0	50.0	58.2	57.8	943-949 bp	1.0	77.1
trnS-GCU-trnC-GCA_p4	ACGGAAAGAGAGGGATTCGA	AAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	58.4	901-907 bp	1.0	76.1
trnS-GCU-trnC-GCA_p5	CTTTCGTCCACTCAGCCATC	GCTTCGTTTGTTGATGAGGC	55.0	50.0	58.6	58.0	880-886 bp	1.0	74.8