Hotspot

rpl22

Genome · 12101-12565 · 465 bp

Back to Gaultheria

Pi 0.0151

MarkerSeek score 50.00

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: Genome
length_bp: 465
variable_sites: 7
pi: 0.0151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	TCCCGGGCATCTACCATTAT	GAACCCGACCCTTTCTGATC	50.0	55.0	58.0	58.0	1233 bp	1.0	80.5
rpl22_p2	CGCCGTCGTAAATAGGAGAG	GAACCCGACCCTTTCTGATC	55.0	55.0	58.0	58.0	1403 bp	1.0	80.4
rpl22_p3	CTCAAAACTGCTCAGCAACG	GAACCCGACCCTTTCTGATC	50.0	55.0	58.0	58.0	1666 bp	1.0	80.3
rpl22_p4	CGCCGTCGTAAATAGGAGAG	CAGCCTGAAACCAAGTGGAT	55.0	50.0	58.0	58.1	877 bp	1.0	80.1
rpl22_p5	CTCAAAACTGCTCAGCAACG	CAGCCTGAAACCAAGTGGAT	50.0	50.0	58.0	58.1	1140 bp	1.0	79.9